Nucleic acid extraction using a homogenizer

All innuSPEED kits are optimally adapted for sample processing using the SpeedMill P12 and hence, permit extremely rapid and very efficient nucleic acid isolation. Both the yield and quality of the isolated nucleic acids are excellent.

 

bs_innuSPEED_Kit

For fast and simple isolation of RNA from bacteria or fungi samples. The kit combines a very efficient homogenization of starting materials and efficient destruction of microbial cell walls using specialized Lysis Tubes which will be processed in the homogenizer SpeedMill P12 or in an other homogenizer. After mechanically processing and heating lysis step the sample will be transferred onto a Spin Filter unit and the DNA will be bound on the Spin Filter surface. A second Spin Filter is used for binding of the RNA. Following by washing and elution of the bound RNA, which is ready to use for further downstream applications.

 

Storage conditions:
The innuSPEED Bacteria/Fungi RNA Kit should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.

Kit components:
Lysis Tube, Lysis Solution, Washing Solutions, RNase-free Water, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No. Amount
845-KS-2510010 10 reactions
845-KS-2510050 50 reactions
845-KS-2510250 250 reactions
 

For isolation of total RNA from different amounts (max. 50 mg) of different kinds of plant material.

 

Storage conditions:
The innuSPEED Plant RNA Kit should be stored dry, at room temperature (14°C - 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.

Kit components:
Lysis Tubes, Lysis Solution, Washing Solution, Rnase-free water, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No. Amount
845-KS-2560010 10 reactions
845-KS-2560050 50 reactions
845-KS-2560250 250 reactions
 

Isolation of RNA from different amounts (up to 20 mg) and different kinds of tissue samples or biopsies.

 

Storage conditions:
The innuSPEED Tissue RNA Kit should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.

Kit components: Lysis Tube, Lysis Solution, Washing Solution, RNase-free Water, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No. Amount
845-KS-2540010 10 reactions
845-KS-2540050 50 reactions
845-KS-2540250 250 reactions
 

For fast and simple isolation of DNA from bacteria or fungi samples. The kit combines a very efficient homogenization of starting materials and efficient destruction of microbial cell walls using specialized Lysis Tubes which will be processed in the homogenizer SpeedMill P12 or in an other homogenizer. After mechanically processing and heating lysis step the sample will be transferred onto a Spin Filter unit and the DNA will be bound on the Spin Filter surface. After washing of the bound DNA the DNA will be eluted from the Spin Filter and is ready to use for further downstream applications.

 

Storage conditions:
The innuSPEED Bacteria/Fungi DNA Kit should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 6 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Store lyophilized Proteinase K at 4°C. Store dissolved Proteinase K at –20°C. Repeated freezing and thawing will reduce the activity dramatically.

Kit components:
Lysis Tube, Lysis Solution, Binding Solution, Proteinase K, Washing Solutions, Elution Buffer, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No. Amount
845-KS-1510010 10 reactions
845-KS-1510050 50 reactions
845-KS-1510250 250 reactions
 

For fast and simple isolation of DNA from soil samples. The kit combines a very efficient homogenization of starting materials and efficient destruction of microbial cell walls using specialized Lysis Tubes which will be processed in the homogenizer SpeedMill P12 or in an other homogenizer. After mechanically processing and heating lysis step the sample will be transferred onto a Spin Filter unit and the DNA will be bound on the Spin Filter surface. After washing of the bound DNA the DNA will be eluted from the Spin Filter and is ready to use for further downstream applications.

 

Storage conditions:
The innuSPEED Soil DNA Kit should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.

Kit components:
Lysis Solutions, Binding Solutions, Washing Solutions, Elution Buffer, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No. Amount
845-KS-1580010 10 reactions
845-KS-1580050 50 reactions
845-KS-1580250 250 reactions
 

For isolation of bacterial DNA from stool samples. The kit combines a very efficient homogenization of starting materials and efficient destruction of bacterial cell walls using specialized Lysis Tubes which will be processed in the homogenizer SpeedMill P12 or in an other homogenizer. After mechanically processing and subsequent proteolytic lysis a pre-filtration step to remove unlysed sample components follows. After pre-filtration the
sample will be transferred onto a Spin Filter unit and the DNA will be bound on the Spin Filter surface. After washing of the bound DNA the DNA will be eluted from the Spin Filter and is ready to use for further downstream applications.

 

Product specification:

Starting material

  • Stool samples from 200 up to 400 mg
  • Stool samples from 200 up to 400 µl

Storage conditions:
The innuSPEED Stool DNA Kit should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.

Kit components:
Lysis Tube, Lysis Solution, Binding Solution, Washing Solutions, Elution Buffer, Pre-filter, Spin Filter, Receiver Tubes, Manual

Order-No. Amount
845-KS-1570010 10 reactions
845-KS-1570050 50 reactions
845-KS-1570250 250 reactions
 

For isolation of genomic DNA from different amounts (up to 100 mg) and different kinds of plant samples.

 

Storage conditions:
The innuSPEED Plant DNA Kit should be stored dry, at room temperature (14°C - 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.Store lyophilized Proteinase K at 4°C. Store dissolved Proteinase K at –20°C. Repeated freezing and thawing will reduce the activity dramatically.

Kit components:
Lysis Tube, Lysis Solution, Binding Solution, Washing Solution, Elution Buffer, Pre-filter, Spin Filter. Receiver Tubes, Elution Tubes, Manual

Order-No. Amount
845-KS-1560010 10 reactions
845-KS-1560050 50 reactions
845-KS-1560250 250 reactions
 

For the isolation of genomic DNA from different amounts (max. 50 mg) of tissue samples or biopsies.

 

Productspecification:

Starting material

  • Tissue up to 50 mg
  • Rodent tail up to 50 mg

Example of use:
Complete homogenization of ticks with the SpeedMill P12 in only 4 minutes and subsequent DNA isolation with the innuSPEED Tissue DNA Kit.

Storage conditions:
The innuSPEED Tissue DNA Kit should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Store lyophilized Proteinase K at 4°C. Store dissolved Proteinase K at –20°C. Repeated freezing and thawing will reduce the activity dramatically.

Kit components:
Lysis Solution, Proteinase K, Reagent Pack A & B, MAG Suspension, MFR Tips, Manual

Order-No. Amount
845-KS-1540010 10 reactions
845-KS-1540050 50 reactions
845-KS-1540250 250 reactions
 

Lysis Tube C is a 0.5 ml tube with a screwing cap containing optimized beads for efficient disruption of plant or animal tissue samples using homogenizers.

 
Order-No. Amount
845-CS-1040050 50 tubes
845-CS-1040100 100 tubes
845-CS-1040250 250 tubes


Further information will follow soon.

 

Lysis Tube B is a 2.0 ml tube with a screwing cap containing optimized beads for efficient disruption of bacteria samples using homogenizers.

 
Order-No.
 Amount
845-CS-1030050 50 tubes
845-CS-1030100 100 tubes
845-CS-1030250 250 tubes


Further information will follow soon.

 

Lysis Tube A is a 2.0 ml tube with a screwing cap containing optimized beads for efficient disruption of plant or animal tissue samples using homogenizers.

 
Order-No. Amount
845-CS-1010050 50 tubes
845-CS-1010100 100 tubes
845-CS-1010250 250 tubes


Further information will follow soon.

 

The Starter Set contains several Lysis Tubes for empirical determination of suitable conditions for the disintegration of samples using homogenizers.

 

Components:
3x Tube A, 3x Tube B, 3x Tube C, 3x Tube P

Order-No. Amount
845-CS-1000012 12 Tubes
 

The Lysis Tube E is a 2.0 ml Tube with screwing cap containing optimized beads for efficient disruption for difficuilt samples as insects, dried tissues or plants using homogenizers.

 
Order-No. Amount
845-CS-1070050 50 tubes
845-CS-1070100 100 tubes
845-CS-1070250 250 tubes


Further information will follow soon.

 

The Lysis Tube P is a 0.5 ml Tube with screwing cap containing optimized beads for efficient disruption for difficuilt samples as insects, dried tissues or plants using homogenizers.

 
Order-No. Amount
845-CS-1020050 50 tubes
845-CS-1020100 100 tubes
845-CS-1020250 250 tubes


Further information will follow soon.

 

Lysis Tube S is a 2.0 ml tube with cap containing special beads using homogenizers.

 
Order-No. Amount
845-CS-1060050 50 tubes
845-CS-1060100 100 tubes
845-CS-1060250 250 tubes


Further information will follow soon.